
Plant Brassinosteroid insensit
ive 1-associated receptor kinase 1 Antibody 植物抗体(Anti-BAK1)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Brassinosteroid insensitive 1-associated receptor kinase 1
- 抗体名:
Anti-BAK1
- 规格:
50 µg
Anti-BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1
AS12 1858| Clonality:Polyclonal| Host:Rabbit| Reactivity:Arabidopsis thaliana, Solanum lycopersicum
Product Info
| Immunogen: | KLH-conjugated peptide, chosen from Arabidopsis thaliana Bak1 sequence, TAIR: | Host: | Rabbit |
| Clonality: | Polyclonal | ||
| Purity: | Immunogen affinity purified serum in PBS pH 7.4. | ||
| Format: | Lyophilized | ||
| Quantity: | 50 µg | ||
| Reconstitution: | For reconstitution add 50 µl of sterile water | ||
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. | ||
| Tested applications: | Immunoprecipitation (IP), Western blot (WB) | ||
| Recommended dilution: | 2 µl/50 µl of Protein G agarose, 1 : 5000 (WB) | ||
| Expected | apparent MW: | 68 | 70 kDa |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana, Solanum lycopersicum |
| Predicted reactivity: | Thelungiella halophila Species of your interest not listed? Contact us |
| Not reactive in: | Hordeum vulgare, Lactuca sativa, Nicotiana benthamiana, Oryza sativa |
Additional Information
Antibodies bind to AtBAK1-Myc. They do not cross react with AtSOBIR. This product can be sold containing proclin if requested. Additional information (application): Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na 2 MoO 4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100. Antibody is reconizing AtBAK1-Myc in total extracts.
Background
BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
a pituitary cDNA encoding a Xenopus 14-3-3 protein. Alignment of the Xenopus protein with known mammalian, Drosophila and plant 14-3-3 polypeptide and with a mammalian protein kinase C inhibitor protein revealed that the neuron-specific 14-3-3-related proteins
Introduction of human 14-3-3 proteins
of the Xenopus intermediate pituitary gland,we cloned and sequenced a pituitary cDNA encoding a Xenopus 14-3-3 protein.Alignment of the Xenopus protein with known mammalian,Drosophila and plant 14-3-3 polypeptide and with a mammalian protein kinase C inhibitor
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