- ￥5850
- Biorbyt
- orb347836
- 英国
- 2026年08月06日
- ELISA, IHC, WB
- Goat
- Rat
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- 文献和实验
- 技术资料
- 抗体名：
Rat IgM (mu chain) Antibody Biotin Conjugated 抗体
- 抗体英文名：
Rat IgM (mu chain) Antibody Biotin Conjugated
- 浓度：
1.0 mg/mL
- 应用范围：
ELISA, IHC, WB
- 宿主：
Goat
- 适应物种：
Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb347836
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
IgG
- 免疫原：
详询
- 规格：
1 mg
产品子类型: Secondary Antibody
描述: Rat IgM (mu chain) antibody (Biotin)
别名: Goat Anti Rat IgM (mu chain) Antibody Biotin Conjugated, Goat Anti-Rat IgM mu chain Antibody Biotin Conjugation
应用稀释比例: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
应用说明: Anti-Rat IgM Biotin conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL
免疫原: Rat IgM whole molecule
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
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文献和实验for potential false-positive results in many tissues. This issue has been appreciated in liver and kidney tissue, but has received very little attention in nervous tissue. To address this concern, we examined the distribution of biotin throughout the rat central
capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC™ RH capture beads
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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