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- 文献和实验
- 技术资料
- 抗体名:
Biotin Monoclonal Antibody 抗体
- 抗体英文名:
Biotin Monoclonal Antibody
- 浓度:
1.0 mg/mL
- 应用范围:
ELISA, FC, IF, IHC, WB
- 宿主:
Mouse
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb344395
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid (sterile filtered)
- 亚型:
IgG2a
- 免疫原:
详询
- 规格:
1 mg
产品子类型: Primary Antibody
描述: Biotin antibody
克隆号: 4C7.D5
别名: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R
应用稀释比例: ELISA: 1:20,000, FC: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF: 2ug/mL, WB: 1:2,000 - 1:10,000
应用说明: Anti-Biotin antibody has been tested in ELISA, western, blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
免疫原: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
保存条件: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.




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文献和实验Coupling of Monoclonal Antibodies with Biotin
The avidin-biotin bond is the strongest known biological interaction between a ligand and a protein ( K d = 1.3 � 10 −15 M at pH 5) ( 1 ). The affinity is so high that the avidin-biotin
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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