Biotin Monoclonal Antibody 抗体,orb344395,Biorbyt产品图

Biotin Monoclonal Antibody 抗体,

orb344395,Biorbyt
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  • ¥37206
  • Biorbyt已认证
  • orb344395
  • 英国
  • 2026年08月05日
  • ELISA, FC, IF, IHC, WB
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Biotin Monoclonal Antibody 抗体

    • 抗体英文名:

      Biotin Monoclonal Antibody

    • 浓度:

      1.0 mg/mL

    • 应用范围:

      ELISA, FC, IF, IHC, WB

    • 宿主:

      Mouse

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb344395

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 形态:

      Liquid (sterile filtered)

    • 亚型:

      IgG2a

    • 免疫原:

      详询

    • 规格:

      1 mg

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Biotin antibody

    克隆号: 4C7.D5

    别名: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R

    应用稀释比例: ELISA: 1:20,000, FC: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF: 2ug/mL, WB: 1:2,000 - 1:10,000

    应用说明: Anti-Biotin antibody has been tested in ELISA, western, blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

    免疫原: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

    防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    纯度: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

    保存条件: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    备注: For research use only.

    产品细节图片1
    Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 1 ug/ml for 45 min at RT. Localization/Staining: plasma membrane and endosome.

    产品细节图片2
    Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2 ug/ml for 45 min at RT. Localization/Staining: plasma membrane and endosome.

    产品细节图片3
    Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2 ug/ml for 45 min at RT. Localization/Staining: plasma membrane and endosome.

    产品细节图片4
    Western Blot of Mouse Anti-Biotin Monoclonal Antibody. Lane 1: Opal PreStained Molecular Weight Marker. Lane 2: Bovine Albumin (BSA) (p/n orb2652777) [20 ng]. Lane 3: Bovine Albumin-Biotin conjugated [20 ng). Primary Antibody: Anti-Biotin at 1.0 µg/ml overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated (p/n orb347506) at 1:40000 for 30 mins at RT. Blocking Buffer: BlockOut Buffer (p/n orb348644) for 1hr at RT. Predicted MW: 75kDa. Exposure: 2sec.

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    图标文献和实验
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