- ￥10764
- Biorbyt
- orb1248019
- 英国
- 2026年07月31日
- ChIP, ELISA, IF, IHC, WB
- Goat
- Human
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- 文献和实验
- 技术资料
- 抗体名：
AR Antibody 抗体
- 抗体英文名：
AR Antibody
- 靶点：
AR
- 浓度：
500 ug/mL
- 应用范围：
ChIP, ELISA, IF, IHC, WB
- 宿主：
Goat
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1248019
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: AR Antibody
别名: AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
应用说明: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5µg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3µg/mL.Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/mL. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
免疫原: The immunogen for this antibody is: EVQLGLGRVYPRPPSC
预测反应性: Bovine, Canine, Mouse, Porcine, Rat
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
特异性: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
研究领域: Cell Biology
UniProt ID: P10275
NCBI 参考序列: NP_000035.2
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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a procedure for the isolation of nondenatured, recombinant human AR with more than 95% purity using four-step chromatography with milligram yields (see Notes 1 -Notes 4 ). Purified AR may be used in physical and biochemical studies, such as monoclonal antibody
Flow Cytometry Assay to Measure Internalization of GPCRs
engineered onto the N-terminus of the GPCR.The following protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.Protocol 1) Grow up receptors in 37degC incubator until confluent
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