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- 文献和实验
- 技术资料
- 抗体名:
CDK2 Antibody 抗体
- 抗体英文名:
CDK2 Antibody
- 靶点:
CDK2
- 浓度:
1 mg/mL
- 应用范围:
IHC, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1253775
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 形态:
Liquid
- 免疫原:
详询
- 规格:
0.1 mg
产品子类型: Primary Antibody
描述: CDK2 Antibody
别名: CDKN2, p33(CDK2), JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, kinase Cdk2, p33 protein kinase
应用说明: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
免疫原: CDK2 (Phospho-Thr160) antibody was raised against a peptide sequence around phosphorylation site of threonine 160 (T-Y-T (p) -H-E) derived from Human CDK2.
分子量: 34 kDa
防腐剂: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
纯化方法: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
特异性: This antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.
研究领域: Epigenetics & Chromatin
UniProt ID: P24941
NCBI 参考序列: NP_001789.2
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


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文献和实验Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
and members of the Bcl-2 protein family. In this chapter, we describe methods for measuring changes in Cdk2 activity during apoptosis. In addition, we also show the details of the generation of an antibody able to immunoprecipitate the cyclin O complexes
spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin α5β1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti
位点的序列,包括肽库和肽芯片 [5,6] 的多种技术可用来鉴定激酶底物。此外,一种 λ 噬菌体 cDNA 表达文库的固相磷酸化筛选 [ 7,8 」以及不同蛋白质相互作用筛选方法,如覆盖方法 [ 9,11] 和酵母双杂交系统 [ 12,14] 已被应用在这方面。最近,蛋白激酶被改造成可接受人工合成的腺苷三磷酸盐(环戊基 ATP ) 模拟物,并用于鉴定特异底物。初步的研究已经证明,通过激酶来研究磷酸化的蛋白质芯片是可行的 [ 18,19] 。为了确定磷酸化位点,抗磷酸化蛋白表位 [6] 的抗体将用于蛋白
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