ELOVL3 Antibody (Biotin) 抗体,orb535147,Biorbyt产品图

ELOVL3 Antibody (Biotin) 抗体,or

b535147,Biorbyt
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  • ¥7605
  • Biorbyt已认证
  • orb535147
  • 英国
  • 2026年07月29日
  • ELISA, IHC, WB
  • Mouse
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      ELOVL3 Antibody (Biotin) 抗体

    • 抗体英文名:

      ELOVL3 Antibody (Biotin)

    • 靶点:

      ELOVL3

    • 浓度:

      1 mg/ml

    • 应用范围:

      ELISA, IHC, WB

    • 宿主:

      Mouse

    • 适应物种:

      Human, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb535147

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Biotin

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgG1

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal antibody against ELOVL3 conjugated to Biotin

    克隆号: V61P2B3-D10

    别名: 3-keto acyl-CoA synthase Elovl3, CIG30, Cold-inducible glycoprotein of 30 kDa, Elongation of very long chain fatty acids protein 3, ELOV3_HUMAN, Elovl3, MGC21435

    应用稀释比例: WB (1:1000)

    应用说明: A 1:1000 dilution was sufficient for detection of Elongation of very long chain fatty acids protein 3 in 10 µg of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    免疫原: Synthetic peptide of Human Elongation of very long chain fatty acids protein 3

    分子量: 40 kDa

    防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

    纯化方法: Protein G Purified

    特异性: Detects 40 kDa.

    研究领域: Metabolism Research

    UniProt ID: Q9HB03

    NCBI 参考序列: NP_689523.1

    Entrez 基因 ID: 83401

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Western Blot analysis of Rat brain showing detection of 31.5 kDa ELOVL3 protein using Mouse Anti-ELOVL3 Monoclonal Antibody, Clone V61P2B3-D10. Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-ELOVL3 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 31.5 kDa. Other Band (s): ~40 kDa, due to PTMs.

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    图标文献和实验
    相关实验
    • The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods

      These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent

    • The Avidin-Biotin Complex(ABC) Method

      , between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin

    • Biotinylation of Antibody

      sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room

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