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- 文献和实验
- 技术资料
- 抗体名:
ELOVL3 Antibody (Biotin) 抗体
- 抗体英文名:
ELOVL3 Antibody (Biotin)
- 靶点:
ELOVL3
- 浓度:
1 mg/ml
- 应用范围:
ELISA, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb535147
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Biotin
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
IgG1
- 免疫原:
详询
- 规格:
100 ug
描述: Mouse monoclonal antibody against ELOVL3 conjugated to Biotin
克隆号: V61P2B3-D10
别名: 3-keto acyl-CoA synthase Elovl3, CIG30, Cold-inducible glycoprotein of 30 kDa, Elongation of very long chain fatty acids protein 3, ELOV3_HUMAN, Elovl3, MGC21435
应用稀释比例: WB (1:1000)
应用说明: A 1:1000 dilution was sufficient for detection of Elongation of very long chain fatty acids protein 3 in 10 µg of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Synthetic peptide of Human Elongation of very long chain fatty acids protein 3
分子量: 40 kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化方法: Protein G Purified
特异性: Detects 40 kDa.
研究领域: Metabolism Research
UniProt ID: Q9HB03
NCBI 参考序列: NP_689523.1
Entrez 基因 ID: 83401
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.

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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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