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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
上海淳麦
- 检测范围：
详见产品标签
- 检测方法：
ELISA
- 适应物种：
人
- 标记物：
HRP
- 样本：
细胞培养上清、血清、血浆、尿液、组织样本等
- 规格：
48T/96T
Human Protein Z ELISA Kit
The Human Protein Z ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of human Protein Z in serum, plasma, cell culture supernatants, urine, and tissue homogenates. This Human Protein Z ELISA Kit provides high sensitivity and specificity for Protein Z measurement, making it an essential tool for coagulation and hemostasis research. The Human Protein Z ELISA Kit utilizes HRP-conjugated detection with TMB substrate for reliable colorimetric readout at 450 nm.
Product Parameters
| Product Name | Human Protein Z ELISA Kit |
| Species | Human |
| Assay Type | Sandwich ELISA |
| Detection Range | 详见产品标签 |
| Sensitivity | 详见产品标签 |
| Sample Type | Serum, plasma, cell culture supernatants, urine, tissue homogenates |
| Conjugate | HRP |
| Standard | Recombinant human Protein Z |
| Format | 96-well plate |
Detection Principle
This Human Protein Z ELISA Kit employs the sandwich ELISA principle. The microplate is pre-coated with a capture antibody specific to human Protein Z. Standards and samples are added to the wells, and Protein Z binds to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin. TMB substrate is added for color development, and the reaction is stopped with stop solution. Absorbance is measured at 450 nm, and the Protein Z concentration is determined from the standard curve.
Operating Procedure
| Step | Operation |
| 1 | Prepare reagents, standards, and samples at room temperature |
| 2 | Add 100 uL of standard or sample to each well, incubate 2 hours at 37°C |
| 3 | Wash plate 3 times with wash buffer |
| 4 | Add 100 uL detection antibody, incubate 1 hour at 37°C |
| 5 | Wash plate 3 times |
| 6 | Add 100 uL HRP-streptavidin, incubate 30 min at 37°C |
| 7 | Wash plate 5 times |
| 8 | Add 90 uL TMB substrate, incubate 15-20 min at 37°C in dark |
| 9 | Add 50 uL stop solution, read absorbance at 450 nm |
Sample Preparation
Serum: Allow blood to clot at room temperature for 2 hours, centrifuge at 1000×g for 20 min, collect supernatant.
Plasma: Centrifuge collected blood at 1000×g for 20 min within 30 min of collection, collect supernatant.
Cell culture supernatant: Centrifuge at 1000×g for 20 min to remove debris.
Tissue homogenate: Homogenize tissue in PBS, centrifuge at 5000×g for 10 min, collect supernatant.
Storage Conditions
Store the Human Protein Z ELISA Kit at 2-8°C. Avoid exposure to light. Reconstituted standards should be used immediately. Do not freeze the kit components.
Application Areas
The Human Protein Z ELISA Kit is widely used in research areas including: coagulation and hemostasis research, thrombosis risk assessment, vitamin K-dependent protein studies, bleeding disorder investigation, anticoagulant therapy monitoring, and hemostatic biomarker analysis.
Keywords: Human Protein Z ELISA Kit, Protein Z detection, human PROZ quantitation, coagulation ELISA, hemostasis ELISA, vitamin K-dependent protein ELISA, human Protein Z immunoassay
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文献和实验1. Kemkes-Matthes K, Matthes KJ. Protein Z deficiency: a new cause of bleeding tendency? Thromb Res. 1995 Dec 1;79(1):49-55. doi: 10.1016/0049-3848(95)00010-5. PMID: 8589158.
2. Yin ZF, Broze GJ Jr. Protein Z. Vitam Horm. 2006;73:185-97. doi: 10.1016/S0083-6729(06)73009-7. PMID: 16806798.
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
through the column. 11. Wash column with 500 ul Buffer HB and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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