IgM Antibody 抗体,orb385797,Biorbyt产品图

IgM Antibody 抗体,orb385797,Bior

byt
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  • ¥4329 - 8775
  • Biorbyt已认证
  • orb385797
  • 英国
  • 2026年07月29日
  • IHC-P, WB
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      IgM Antibody 抗体

    • 抗体英文名:

      IgM Antibody

    • 应用范围:

      IHC-P, WB

    • 宿主:

      Mouse

    • 适应物种:

      Human

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb385797

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      Mouse IgG1, kappa

    • 免疫原:

      详询

    • 规格:

      20 ug/100 ug

    规格:20 ug产品价格:¥4329.0
    规格:100 ug产品价格:¥8775.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.

    克隆号: MuHC2

    应用稀释比例: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    应用说明: Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    免疫原: Human Mu heavy chain was used as the immunogen for this IgM antibody.

    防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

    纯化方法: Protein G

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of IgM antibody and Raji cell lysate (clone MuHC2). Expected molecular weight: 70-75 kDa.

    产品细节图片2
    IHC analysis of FFPE human tonsil tissue and IgM antibody (clone MuHC2). Required HIER: boil sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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    图标文献和实验
    相关实验
    • Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays

      criteria must be met. Laboratory testing to identify aPL antibodies includes lupus anticoagulant (liquid-based clotting assays) and immunological solid-phase assays (usually enzyme-linked immunosorbent assay formats) for IgG and/or IgM anticardiolipin (aCL

    • 抗体(antibody)

      。此时若再次接触抗原,可使已消失的抗体快速上升。如再次刺激机体的抗原与初次相同,则称为特异性回忆反应;若与初次反应不同,则称为非特异性回忆反应。非特异性回忆反应引起的抗体的上升是暂时性的,短时间内即很快下降。   抗体的分类 (1)按作用对象,可将其分为抗毒素、抗菌抗体、抗病素抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上)。(2)按理化性质和生物学功能,可将其分为IgG、IgA、IgM、IgE、IgD五类。(3)按与抗原结合后是否出现

    • Protein A Purification of Antibody

      PBS, overnight, 2-8℃.Re-equilibrate column with Binding Buffer. Store column at 2-8℃.Determine protein concentration of Ab, aliquot, and store at -20℃.3. NoteExtinction Coefficient for IgG = 1.4 and for IgM = 1.2

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