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- 文献和实验
- 技术资料
- 抗体名:
b-Catenin Antibody 抗体
- 抗体英文名:
b-Catenin Antibody
- 应用范围:
FACS, IF, IHC-P, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb385991
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
Mouse IgG1, kappa
- 免疫原:
详询
- 规格:
20 ug/100 ug
| 规格: | 20 ug | 产品价格: | ¥4329.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥8775.0 |
产品子类型: Primary Antibody
描述: Beta Catenin is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. b-Catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. b-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]
克隆号: 6F9
应用稀释比例: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
应用说明: Optimal dilution of the b-Catenin antibody to be determined by the researcher.
免疫原: Chicken beta-Catenin was used as the immunogen for the b-Catenin antibody.
防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways, Wnt
UniProt ID: P35222
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.



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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
Systemor equivalent). 4. Nitrocellulose or polyvinyl difluoride (PVDF) membrane. 5. Wnt3A antibody (available from R&D Systems, Minneapolis,MN). 6. Appropriate conjugated secondary antibody for detection. 2.4.2. b-Catenin Stabilization
The effect of lithium chloride on Lytechinus variegates embryos
at the completion of gastrulation (Cameron, 1997). Veg2 and micromere lineages normally contribute to secondary mesenchyme, the coelomic sacs, as well as most of the archenteron (Cameron, 1997). High levels of B-catenin in micromeres prior to gastrulation suggest
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