ATG4B Antibody (FITC) 抗体,orb383938,Biorbyt产品图

ATG4B Antibody (FITC) 抗体,orb38

3938,Biorbyt
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  • ¥7605
  • Biorbyt已认证
  • orb383938
  • 英国
  • 2026年07月29日
  • ICC, IF, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      ATG4B Antibody (FITC) 抗体

    • 抗体英文名:

      ATG4B Antibody (FITC)

    • 靶点:

      ATG4B

    • 浓度:

      1 mg/ml

    • 应用范围:

      ICC, IF, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Mouse

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb383938

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      FITC

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Rabbit polyclonal antibody against ATG4B conjugated to FITC.

    别名: ATG4B, APG4B, AUTL1, KIAA0943, ATG4B_HUMAN, Autophagy-related protein 4 homolog B, Autophagin-1, Autophagin 1, Autophagy-related cysteine endopeptidase 1, AUT-like 1 cysteine endopeptidase, Cysteine protease ATG4B, APG4 autophagy 4 homolog B, ATG4 autophagy related 4 homolog B (S. cerevisiae), EC:3.4.22.-, hAPG4B, MGC1353

    应用稀释比例: WB (1:1000), ICC/IF (1:100)

    应用说明: A 1:1000 dilution was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    免疫原: Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)

    分子量: 44 kDa

    防腐剂: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化方法: Peptide Affinity Purified

    特异性: Detects ~44 kDa.

    研究领域: Cell Biology

    UniProt ID: Q9Y4P1

    NCBI 参考序列: NP_037457.3

    Entrez 基因 ID: 23192

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.

    产品细节图片2
    Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band (s): Band appears at ~40 kDa.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

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