- ￥6552
- Biorbyt
- orb151598
- 英国
- 2026年07月29日
- ICC, IF, IHC
- Rabbit
- Human
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- 文献和实验
- 技术资料
- 抗体名：
CDC37 Antibody (Biotin) 抗体
- 抗体英文名：
CDC37 Antibody (Biotin)
- 靶点：
CDC37
- 浓度：
1.68 mg/ml
- 应用范围：
ICC, IF, IHC
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb151598
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ug
描述: Rabbit polyclonal to CDC37 (Biotin). Hsp90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies.
别名: CDC37, Hsp90 co-chaperone Cdc37, Cell division control protein 37, Hsp90 chaperone protein kinase-targeting subunit, CDC37_HUMAN, CDC37A, p50, p50Cdc37, S. cerevisiae hypothetical protein CDC37
应用稀释比例: WB (1:2000), ICC/IF (1:200)
应用说明: A 1:2000 dilution was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
免疫原: Native human Cdc37, full length
分子量: 44.5kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
纯化方法: Protein A Purified
特异性: Detects ~44.5kDa.
研究领域: Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction
UniProt ID: Q16543
NCBI 参考序列: NP_008996.1
Entrez 基因 ID: 11140
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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