- ￥8775
- Biorbyt
- orb396692
- 英国
- 2026年07月24日
- ELISA, FACS, FC, ICC, IF, IHC, WB
- Mouse
- All
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- 文献和实验
- 技术资料
- 抗体名：
Methylglyoxal Antibody (RPE) 抗体
- 抗体英文名：
Methylglyoxal Antibody (RPE)
- 靶点：
Methylglyoxal
- 浓度：
1 mg/ml
- 应用范围：
ELISA, FACS, FC, ICC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
All
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb396692
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
RPE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG2a
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
描述: Mouse monoclonal antibody against Methylglyoxal conjugated to RPE .
克隆号: 90000000
别名: Methylglyoxal, 2-Oxopropanal, 2 oxo propanal, MG, Pyruvaldehyde, Methylglyoxal (MG), MG-modified protein
应用稀释比例: WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); IHC (1:50)
应用说明: A 1:1000 dilution was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
免疫原: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
纯化方法: Protein G Purified
特异性: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
研究领域: Metabolism Research
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
描述: Mouse monoclonal antibody against Methylglyoxal conjugated to RPE .
克隆号: 90000000
别名: Methylglyoxal, 2-Oxopropanal, 2 oxo propanal, MG, Pyruvaldehyde, Methylglyoxal (MG), MG-modified protein
应用稀释比例: WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); IHC (1:50)
应用说明: A 1:1000 dilution was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
免疫原: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
纯化方法: Protein G Purified
特异性: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
研究领域: Metabolism Research
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) Methylglyoxal Antibody. (G, H) Composite.
Immunohistochemistry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:25 for 3 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.
Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 μM H2O2 for 24 hours.
Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
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文献和实验相关实验
different tissues of the eye, i.e., cornea, lens, iris, retina, optic nerve, retinal pigment epithelium (RPE), and sclera. We further describe in detail how to process these eye samples in order to obtain high?quality RNA for RNA expression profiling studies
基本没有RPE原有性质特征，只具有成纤维细胞的特征。 【qkk1229】同意N代后就是成纤维细胞的模样，很多细胞体外培养到后来，都长的象成纤维细胞，不知道为什么，有人知道吗？ 【jamesxia】去除成纤维细胞 1、反复贴壁法（差速贴壁法）：倒去旧培养液，用Hank′s液洗3次，然后加入消化液（0.2%EDTA+0.25%胰蛋白酶），37℃10min，镜下可见成纤维细胞成圆形，癌细胞维持原状。吸出消化液，加Hank′s液吹打细胞使成纤维细胞脱落，吸出旧液，组织块和癌细胞团用Hank′s液轻轻
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Methylglyoxal Antibody (RPE) 抗体，orb396692，Biorbyt
￥8775
