Acrolein Antibody (FITC) 抗体,orb396472,Biorbyt产品图

Acrolein Antibody (FITC) 抗体,or

b396472,Biorbyt
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  • ¥8658
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  • orb396472
  • 英国
  • 2026年07月24日
  • ELISA, FACS, FC, ICC, IF, WB
  • Mouse
  • All
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Acrolein Antibody (FITC) 抗体

    • 抗体英文名

      Acrolein Antibody (FITC)

    • 靶点

      Acrolein

    • 浓度

      1 mg/ml

    • 应用范围

      ELISA, FACS, FC, ICC, IF, WB

    • 宿主

      Mouse

    • 适应物种

      All

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb396472

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      FITC

    • 克隆性

      Monoclonal

    • 保存条件

      详询

    • 亚型

      IgG1

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal antibody against Acrolein conjugated to FITC.

    克隆号: 2H2

    别名: Acraldehyde, Acrylaldehyde, Acrylic aldehyde, Allyl aldehyde, Propenal, 2-Propenal, Prop-2-enal, Prop-2-en-1-al, Acroleine, Paracetaldehyde, Acquinite, Aqualin, Aqualine, Biocide

    应用稀释比例: WB (1:1000); ICC/IF (1:50); ELISA (1:1000)

    应用说明: A 1:1000 dilution was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    免疫原: Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

    防腐剂: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化方法: Protein G Purified

    特异性: Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

    研究领域: Cancer Biology, Cell Biology, Metabolism Research, Neuroscience

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) Acrolein Antibody. (G, H) Composite.

    产品细节图片2
    Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 μg). Lane 3: Acrolein-BSA (2.0 μg). Lane 4: BSA (0.5 μg). Lane 5: BSA (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

    产品细节图片3
    Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 μM H2O2 for 24 hours.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

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