- ￥6552
- Biorbyt
- orb22532
- 英国
- 2026年07月22日
- ELISA, WB
- Rat
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- 文献和实验
- 技术资料
- 抗体名：
Goat anti-Transcortin / CBG (rat) Antibody 抗体
- 抗体英文名：
Goat anti-Transcortin / CBG (rat) Antibody
- 靶点：
Transcortin / CBG (rat)
- 应用范围：
ELISA, WB
- 适应物种：
Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb22532
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ug
描述: Goat Polyclonal to CBG
别名: anti -transcortin antibody, anti-serpin A6 antibody, anti-serine (or cysteine) proteinase inhibitor antibody, anti-clade A antibody, anti-(alpha-1 antiproteinase antibody, anti-antitrypsin) member 6 antibody, anti-corticosteroid-binding globulin antibody, anti-corticosteroid binding globulin antibody, anti-Cbg antibody, anti-MGC112780 antibody, anti-serine (or cysteine) peptidase inhibitor antibody, anti-clade A antibody, anti-member 6 antibody, anti-Serpina6 antibody
应用稀释比例: ELISA: 1:16000, WB: 0.03-0.1 μg/ml
应用说明: WB: Approx 55kDa band observed in Rat Liver lysates (calculated MW of 44.7kDa according to NP_001009663.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Richard et al, Endocrinology. 2010 Feb;151(2):649-59; PMID: 20022933). Recommended concentration: 0.03-0.1 μg/ml.
分子量: 44.7
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
蛋白序列: KASQQINQHVKDKT
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RRID: AB_10940893
研究领域: Cell Biology
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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