- ￥6552
- Biorbyt
- orb18316
- 英国
- 2026年07月22日
- ELISA, IP, WB
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Goat anti-Aurora kinase A / AURKA Antibody 抗体
- 抗体英文名：
Goat anti-Aurora kinase A / AURKA Antibody
- 靶点：
Aurora kinase A / AURKA
- 应用范围：
ELISA, IP, WB
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb18316
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ug
描述: Goat polyclonal antibody to AURKA
别名: anti AURKA antibody, anti aurora kinase A antibody, anti AIK antibody, anti ARK1 antibody, anti AURA antibody, anti AURORA2 antibody, anti BTAK antibody, anti MGC34538 antibody, anti STK15 antibody, anti STK6 antibody, anti STK7 antibody, anti IPL1-related kinase antibody, anti aurora-A antibody, anti aurora-related kinase 1 antibody, anti aurora/IPL1-like kinase antibody, anti breast-tumor-amplified kinase antibody, anti serine/threonine kinase 15 antibody, anti serine/threonine kinase 6 antibody, anti serine/threonine protein kinase 15 antibody, anti serine/threonine protein kinase 6 antibody, anti OTTHUMP00000031340 antibody, anti OTTHUMP00000031342 antibody, anti OTTHUMP00000031343 antibody, anti OTTHUMP00000031344 antibody, anti OTTHUMP00000031345 antibody, anti OTTHUMP00000166071 antibody
应用稀释比例: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
应用说明: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 μg/ml.
分子量: 45.8
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
蛋白序列: QNKESASKQS
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RRID: AB_10746535
研究领域: Cell Biology
Entrez 基因 ID: 6790
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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