- ￥1800
- Proteintech
- KE00162-96T
- 2026年07月20日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州市左克生物
- 适应物种：
Human
- 规格：
96T
ProductInformation
KE00162isasolidphasesandwichEnzymeLinked-Immuno-SorbentAssay(SandwichELISA).TheTIMP2ELISAkitistobeusedtodetectandquantifyproteinlevelsofendogenousTIMP2.TheassayrecognizeshumanTIMP2.AnantibodyspecificforTIMP2hasbeenpre-coatedontothemicrowells.TheTIMP2proteininsamplesiscapturedbythecoatedantibodyafterincubation.Followingextensivewashing,anotherantibodyofbiotinylatedspecificforhumanTIMP2isaddedtodetectthecapturedhumanTIMP2protein.Forsignaldevelopment,Streptavidin-HRPisadded,followedbyTetramethyl-benzidine(TMB)reagent.Solutioncontainingsulfuricacidisusedtostopcolordevelopmentandthecolorintensitywhichisproportionaltothequantityofboundproteinismeasurableat450nmwiththecorrectionwavelengthsetat630nm.
Productname HumanTIMP2ELISAKit Tests 1X96wellplate Sampletype Serum,Plasma,Cellculturesupernatants,Urine,Saliva备注：对未验证样本，我们提供技术支持和售后保障。 Assaytype Sandwich Sensitivity 0.3pg/mL Range 31.25-2000pg/mL Reactivity Human Testedapplications SandwichELISA GeneID(NCBI) 7077
属性：
BackgroundInformation
TIMP2,a21-kDaprotein,isamemberofthefamilyofmatrixmetalloproteinaseinhibitors,whichcontainsfourmembers(TIMP1,TIMP2,TIMP3,andTIMP4).Tissueinhibitorsofmetalloproteinases(TIMPs)aremultifacetedmoleculesthatexhibitpropertiesbeyondtheirclassicalproteinaseinhibitoryfunction.TIMP-2istheonlyTIMPfunctioningasbothanMMPinhibitorandanactivator,becauseitcaninteractwithmembranetype1MMPandpro-MMP2tofacilitatetheactivationofpro-MMP2toitsactiveform.TIMP2canregulatemanyphysiologicalprogressionsuchastumorgrowth,metastasis,andangiogenesisthroughmatrixmetalloproteinases(MMPs).TIMP2knockoutresultedinconstitutiveMMP9activationabundantlyandadverseMIremodelinginvivo.
引用文献数：
1
常见问题解答：
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2uL人血清样本，加入198uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL100倍稀释的人血清样本+198uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL10000倍稀释的人血清样本+198uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.
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