Galanin Rabbit Polyclonal Antibody 抗体,orb402208,Biorbyt产品图

Galanin Rabbit Polyclonal Anti

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  • ¥5499
  • Biorbyt已认证
  • orb402208
  • 英国
  • 2026年07月22日
  • WB
  • Rabbit
  • Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Galanin Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      Galanin Rabbit Polyclonal Antibody

    • 靶点

      Galanin peptides [Cleaved into: Galanin; Galanin message-associated peptide

    • 浓度

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围

      WB

    • 宿主

      Rabbit

    • 适应物种

      Mouse, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb402208

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Lyophilized

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-Galanin Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.

    别名: Galanin peptides; Galanin; Galanin message-associated peptide; GMAP; GAL; GAL1; GALN; GLNN

    应用稀释比例: Western blot, 0.1-0.5μg/ml

    免疫原: A synthetic peptide corresponding to a sequence in the middle region of human Galanin, which shares 86.2% amino acid (aa) sequence identity with both mouse and rat Galanin.

    交叉反应: No cross-reactivity with other proteins.

    分子量: 19 kDa

    防腐剂: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

    特异性: No cross reactivity with other proteins.

    研究领域: Epigenetics & Chromatin

    UniProt ID: P22466

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of Galanin using anti-Galanin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galanin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galanin at approximately 19KD. The expected band size for Galanin is at 13KD.

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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

      Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed        

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Generation and Selection of Rabbit Antibody Libraries by Phage Display

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