RENT1/hUPF1 Rabbit Polyclonal

Antibody 抗体，orb334574，Biorbyt

价格￥5499

品牌Biorbyt
货号orb334574
产地英国
更新日期2026年07月22日

应用范围FC, ICC, IF, IHC, WB
宿主Rabbit
适应物种Human, Mouse, Rat

13 年钻石会员

相关产品推荐更多 >

万千商家帮你免费找货

0 人在求购买到急需产品

详细信息
文献和实验
技术资料

抗体名：
RENT1/hUPF1 Rabbit Polyclonal Antibody 抗体
抗体英文名：
RENT1/hUPF1 Rabbit Polyclonal Antibody
靶点：
Regulator of nonsense transcripts 1
浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围：
FC, ICC, IF, IHC, WB
宿主：
Rabbit
适应物种：
Human, Mouse, Rat
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb334574
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Lyophilized
亚型：
Rabbit IgG
免疫原：
详询
规格：
100 ug

类别: Antibodies

产品子类型: Primary Antibody

描述: RENT1/hUPF1 Rabbit Polyclonal Antibody

别名: Regulator of nonsense transcripts 1; 3.6.4.-; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1; UPF1; KIAA0221, RENT1

应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human

免疫原: A synthetic peptide corresponding to a sequence in the middle region of human RENT1/hUPF1, identical to the related mouse and rat sequences.

交叉反应: No cross-reactivity with other proteins

分子量: 140 kDa

防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

纯化方法: Immunogen affinity purified.

复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

特异性: No cross reactivity with other proteins.

研究领域: Epigenetics & Chromatin, Molecular Biology

UniProt ID: Q92900

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

Flow Cytometry analysis of PC-3 cells using anti-RENT1/hUPF1 antibody. Overlay histogram showing PC-3 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RENT1/hUPF1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IF analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-RENT1/hUPF1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-RENT1/hUPF1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-RENT1/hUPF1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-RENT1/hUPF1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RENT1/hUPF1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RENT1/hUPF1 at approximately 140 kDa. The expected band size for RENT1/hUPF1 is at 124 kDa.

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

文献和实验

大家都在搜

大家都在搜