OGT/O-Linked N-Acetylglucosami
ne Transferase Rabbit Polyclonal Antibody 抗体，orb334499，Biorbyt- ￥5499
- Biorbyt
- orb334499
- 英国
- 2026年07月22日
- FC, ICC, IF, IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
OGT/O-Linked N-Acetylglucosamine Transferase Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
OGT/O-Linked N-Acetylglucosamine Transferase Rabbit Polyclonal Antibody
- 靶点：
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
- 浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围：
FC, ICC, IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb334499
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: OGT/O-Linked N-Acetylglucosamine Transferase Rabbit Polyclonal Antibody
别名: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; 2.4.1.255; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; OGT
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human, Mouse
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human OGT, identical to the related mouse and rat sequences.
交叉反应: No cross-reactivity with other proteins.
分子量: 117 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Epigenetics & Chromatin; Pharmacology & Drug Discovery, Neuroscience, Protein Biochemistry, Signal Transduction
UniProt ID: O15294
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: OGT/O-Linked N-Acetylglucosamine Transferase Rabbit Polyclonal Antibody
别名: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; 2.4.1.255; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; OGT
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human, Mouse
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human OGT, identical to the related mouse and rat sequences.
交叉反应: No cross-reactivity with other proteins.
分子量: 117 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Epigenetics & Chromatin; Pharmacology & Drug Discovery, Neuroscience, Protein Biochemistry, Signal Transduction
UniProt ID: O15294
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Flow Cytometry analysis of RAW264.7 cells using anti-OGT antibody. Overlay histogram showing RAW264.7 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGT Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U937 cells using anti-OGT antibody. Overlay histogram showing U937 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGT Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of OGT using anti-OGT antibody. OGT was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-OGT Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of OGT using anti-OGT antibody. OGT was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-OGT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of OGT using anti-OGT antibody. OGT was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-OGT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of OGT using anti-OGT antibody. OGT was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-OGT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of OGT using anti-OGT antibody. OGT was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-OGT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of OGT using anti-OGT antibody. OGT was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-OGT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of OGT using anti-OGT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OGT at approximately 117 KD. The expected band size for OGT is at 117 KD.
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文献和实验相关实验
Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Polyclonal and Monoclonal Antibodies（克隆和克隆抗体）
at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method
around the phosphorylation site of serine 33 (L-D-SP-G-I)。 http://www.genscript.com/product_001/rec_antibody/code/A00584/category/antibody/Rabbit_Anti_beta-Catenin_Ab-33_polyclonal.html bettyzyj http://www.signalwayantibody.com/resurgam/uppdf/21211.pdf
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OGT/O-Linked N-Acetylglucosamine Transferase Rabbit Polyclonal Antibody 抗体，orb334499，Biorbyt
￥5499
