Kv1.4/KCNA4 Rabbit Polyclonal

Antibody 抗体，orb334539，Biorbyt

价格￥5499

品牌Biorbyt
货号orb334539
产地英国
更新日期2026年07月18日

应用范围IHC, WB
宿主Rabbit
适应物种Human

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抗体名：
Kv1.4/KCNA4 Rabbit Polyclonal Antibody 抗体
抗体英文名：
Kv1.4/KCNA4 Rabbit Polyclonal Antibody
靶点：
Potassium voltage-gated channel subfamily A member 4
浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围：
IHC, WB
宿主：
Rabbit
适应物种：
Human
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb334539
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Lyophilized
亚型：
Rabbit IgG
免疫原：
详询
规格：
100 ug

类别: Antibodies

产品子类型: Primary Antibody

描述: Kv1.4/KCNA4 Rabbit Polyclonal Antibody

别名: Potassium voltage-gated channel subfamily A member 4; HPCN2; Voltage-gated K (+) channel HuKII; Voltage-gated potassium channel HBK4; Voltage-gated potassium channel HK1; Voltage-gated potassium channel subunit Kv1.4; KCNA4; KCNA4L

应用稀释比例: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Western blot, 0.1-0.5μg/ml, Human

免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human Kv1.4, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

交叉反应: No cross-reactivity with other proteins.

分子量: 73 kDa

防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

纯化方法: Immunogen affinity purified.

复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

特异性: No cross reactivity with other proteins.

研究领域: Epigenetics & Chromatin

UniProt ID: P22459

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

IHC analysis of Kv1.4 using anti-Kv1.4 antibody. Kv1.4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Kv1.4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of Kv1.4 using anti-Kv1.4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: HT1080 Whole Cell Lysate, Lane 4: PANC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Kv1.4 at approximately 73 kDa. The expected band size for Kv1.4 is at 73 kDa.

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