VRK1 Rabbit Polyclonal Antibody 抗体,orb334640,Biorbyt产品图

VRK1 Rabbit Polyclonal Antibod

y 抗体,orb334640,Biorbyt
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  • ¥5499
  • Biorbyt已认证
  • orb334640
  • 英国
  • 2026年07月22日
  • WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      VRK1 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名:

      VRK1 Rabbit Polyclonal Antibody

    • 靶点:

      Serine/threonine-protein kinase VRK1

    • 浓度:

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围:

      WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb334640

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Lyophilized

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-VRK1 Antibody. Tested in WB applications. This antibody reacts with Human, Rat.

    别名: Serine/threonine-protein kinase VRK1; 2.7.11.1; Vaccinia-related kinase 1; VRK1

    应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Rat

    免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human VRK1, different from the related mouse sequence by three amino acids.

    交叉反应: No cross-reactivity with other proteins.

    分子量: 55 kDa

    防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

    特异性: No cross reactivity with other proteins.

    研究领域: Cell Biology, Epigenetics & Chromatin, Neuroscience, Protein Biochemistry, Signal Transduction

    UniProt ID: Q99986

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of VRK1 using anti-VRK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VRK1 at approximately 55 kDa. The expected band size for VRK1 is at 45 kDa.

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