CleanFc™ Rabbit Anti-Human CD8

Monoclonal antibody, clone TL2 [PE/Cy5]

15 年钻石会员

抗体名：
CD8 Antibody
抗体英文名：
CleanFc™ Rabbit Anti-Human CD8 Monoclonal antibody, clone TL2 [PE/Cy5]
靶点：
CD8
浓度：
Lot specific
应用范围：
FC
宿主：
Rabbit
适应物种：
Human
保质期：
See COA
抗原来源：
Native Protein
级别：
RUO
库存：
In Stock
标记物：
PE/Cy5
克隆性：
Monoclonal
保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
形态：
Liquid
亚型：
IgG
免疫原：
Human peripheral blood T lymphocytes
规格：
100 Tests

CleanFc™ Rabbit Anti-Human CD8 Monoclonal antibody for FC

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文献和实验

Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
实验原理 A mixture of biotinylated monoclonal antibodies against the non-CD8 T cells is added to the starting sample and allowed to bind to the cells. Depletion MyOne™ SA Dynabeads® are added and will bind to the antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend

CleanFc™ Rabbit Anti-Human CD8 Monoclonal antibody, clone TL2 [PE/Cy5]

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