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- 文献和实验
- 技术资料
- 抗体名:
Anti-MBP MAb
- 抗体英文名:
Anti-MBP monoclonal antibody, clone TNJ 00
- 靶点:
MBP
- 应用范围:
IHC-Fr, ELISA, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
See COA
- 抗原来源:
Recombinant Protein
- 级别:
RUO
- 库存:
In Stock
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
store at -20°C. Avoid freeze / thaw cycles.
- 形态:
Liquid
- 亚型:
IgG2b
- 免疫原:
Recombinant fragment: Ala-Ser-Asp-Tyr-Lys-Ser, corresponding to amino acids 131-136 of Human Myelin Basic Protein
- 规格:
100 μl
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
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