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文献和实验nanoparticles [J].Bioconjug Chem, 2002 13(2): 264-8. 14 Polyakov V, Sharma V, Dahlheimer JL, et al. Novel Tat-peptide chelates for direct transduction of technetium-99m and rhenium into human cells for imaging and radiotherapy [J]. Bioconjug Chem, 2000, 11
【共享】流式 Overview of Flow Cytometry
, it will be unacceptable in an experiment requiring 3 × 107 cells of 99.9% purity.With ECS, expensive instrumentation is used to separate cells one cell at a time. The only way to increase cell yield/unit time is to increase the number of flow cytometers used. In contrast
centrifuge. Total RNA was precipitated with 2 vol of 2-propanol (Fluka) at −20°C for at least 16 h. The RNA pellet was washed with 75% ethanol (Merck), dried, and dissolved in DEPC-treated water (Ambion). The RNA was quantified with Quant-iT™ RiboGreen® RNA
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