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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
for detection of the FISH signals BSA (Sigma, 98% electrophoresis purity) Normal donkey or goat serum (Jackson laboratories) Blocking solution: PBS 1X; 0.3% Triton X-100; 1% BSA; 10% normal donkey or goat serum FITC-anti-Biotin
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
Wash in PBS at room temperature for 5 minutes. Detection of the Immunostaining Add 20µl of the diluted Cy3-Anti-Rabbit secondary antibody (1/700e in blocking solution + 2% normal goat serum). Cover with a coverslip and incubate
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