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- 北京安必奇
- DFCO-L044
- 2026年07月14日
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Store at 2-8°C. Keep refrigerated. Do not freeze. Keep container closed and fluorescent particles protected from light.
- 规格：
1mL
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文献和实验Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands
, and addition of amino, carboxyl, thiophosphate, phosphate, and masked thiol groups at the 5 terminus of an oligodeoxyribonucleotide are described elsewhere in the series. This unit reports procedures for coupling the ligands and oligonucleotides
centrifuge. Total RNA was precipitated with 2 vol of 2-propanol (Fluka) at −20°C for at least 16 h. The RNA pellet was washed with 75% ethanol (Merck), dried, and dissolved in DEPC-treated water (Ambion). The RNA was quantified with Quant-iT™ RiboGreen® RNA
Synthesis and Probing of Membrane-bound Peptide Arrays
from their carboxyl termini using Fmoc-amino acid derivatives. After completion of the synthesis and cleavage of all side-chain protecting groups, the peptide array on the membrane is incubated with the potential interaction partners to identify their target sequences
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