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文献和实验Vybrant® DyeCycle Green and Orange Stains
Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
blood sample into Processing Plate (supplied) followed by adding 10 ul Proteinase K solution and 10 ul Mag-Bind™ Particle R. Note: Proteinase K and Mag-Bind™ Particles R can be prepared as master mix and add to each at one time. For 96 samples, mix
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
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