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- 文献和实验
- 技术资料
- 应用:
The Avian Influenza Virus Subtype H5N2 Real Time PCR Kit is an in vitro test, based on real-time PCR technology, for the detection of H5N2 Virus RNA. Samples can be obtained from human throat swabs and nasopharyngeal secretions.
- 样本:
Human throat swab, nasopharyngeal secretions
- 规格:
50 Reactions
Outbreaks of "high-path" H5N2 result in the culling of thousands of birds in poultry farms from time to time. It appears that people who work with birds can be infected by the virus but suffer hardly any noticeable health effects. Even people exposed to the highly pathogenic H5N2 variety that killed ostrich chicks in South Africa only seem to have developed conjunctivitis, or perhaps a mild respiratory illness. There is no evidence of the human-to-human spread of H5N2. In 1983, H5N2 caused flu outbreaks in chickens and turkeys on many farms, which led to significant economic losses.
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文献和实验. Measurement of cytokine induction, based on the design of primers and probes for RT-PCR or real-time RT-PCR for the cytokine mRNA, has become one of the more recent technologies reported to measure avian cytokines. It is important to note that small nucleotide
Avian Influenza Virus Sample Types, Collection, and Handling
. The diagnostic tests to be performed should be considered prior to sample collection. Sera are acceptable samples for ELISA or agar gel precipitin tests, but not for real-time RT-PCR. Likewise, swabs and/or tissues are acceptable for real-time RT-PCR and virus
Detection and Identification of the H5 Hemagglutinin Subtype by Real-Time RT-PCR
priority worldwide, the procedure for real-time RT-PCR (rRT-PCR) identification of the H5 H subtype is presented here. This assay can identify the H5 hemagglutinin of any genetic lineage (North American or Asian) and either pathotype (highly pathogenic
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