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- 详细信息
- 文献和实验
- 技术资料
- 应用:
IP, CoIP
- 标记物:
Anti-Ubiquitin Single Domain Antibody
- 规格:
20reactions
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文献和实验Mag-Bind® Plasmid Mega Magnetic Protocol
few times. 9. Incubate for 10 minutes at room temperature, mixing few times by inverting the bottle. 10. Magnetize the magnetic particles with a magnet until liquid is cleared. 11. Add 70 mL of SPM Wash Buffe diluted with absolute ethanolr
E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
to each sample. Mix by vortexing at maxi speed for 30 seconds. 3. Incubate sample at 65°C for 15 min. Briefly vortexing the tube during incubation. 4. Add 150 ul absolute ethanol and 10 ul Mag-Bind Particles Solution C to the sample. Mix by vortexing
Single and Double FISH protocols for Drosophila
. 2. PBT solution: 1XPBS, 0.1% Tween-20. 3. PBTB solution: 1XPBS, 0.1% Tween-20 and 0.5% milk powder. 4. Mouse monoclonal anti-fluorescein antibody (IgG: 1/2000 dilution of a 0.1 mg/ml stock solution [see notes 2,3]; Boehringer Mannheim, Laval
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