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文献和实验Inducing RNAi with siRNA Cocktails Generated by RNase III
a number of long dsRNA substrates. Human GAPDH, La, and c-FOS dsRNA (200 bp) was prepared by in vitro transcription ( Silencer ™ siRNA Cocktail Kit (RNase III); See Materials and Methods). The dsRNA was digested using 1 U RNase III
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
Assay Kit provides:Calcein AMMitoTracker Red CMXRos, a red-fluorescent mitochondrial stain (excitation/emission maxima ~579/599 nm)Hoechst 33342, a blue-fluorescent nuclear stain (excitation/emission maxima ~350/461 nm)IonomycinCoCl2Dimethylsulfoxide
]. After removal of unbound cells with PBS, attached cells were treated with 0.1% crystal violet for 15 minutes. The OD value at 540 nm was measured by a Spectramax 250 microplate reader.2.12. Flow cytometric analysisSurface levels of CD80, CD86, CD29, and CD
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