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- 文献和实验
- 技术资料
- 库存：
In Stock
- 检测方法：
Competitive ELISA
- 应用：
Quantitative
- 适应物种：
Human
- 样本：
serum
- 规格：
96T
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文献和实验Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids
Upon infection with Epstein-Barr virus (EBV), primary human B-lymphocytes are efficiently immortalized and give rise to lymphoblastoid cell lines in vitro. Four of the 11 viral genes expressed in the immortalized B cells
Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids
Plasmids containing the Epstein-Barr virus (EBV) latent origin of replication, OriP, are stably maintained in human cells expressing the viral EBNA-1 protein. This stable maintenance is owing to the ability of EBNA-1 to activate DNA
The method described in this chapter uses limiting dilution analysis in conjunction with RT-PCR to determine quantitatively what percentage of EBV-infected cells within a given population are expressing the viral genes EBNA-1 Q-K, EBNA
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