3,3′-Diethyloxacarbocyanine, I

Flow Cytometric Methods for Sperm Assessment

) for assessing sperm viability; the double stain PNA-FITC (peanut agglutinin-fluorescein isothiocyanate) and PI for assessing acrosomal integrity combined with sperm viability; and JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolylcarbocyanine iodide

Analysis of Cell Cycle by Flow Cytometry

Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4′,6′-diamidino

Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen

to resorufin which generates a fluorescent signal. The mitochondrial membrane potential was quantified by determining the cellular uptake of a fluorescent dye, (5,5′ ,6,6′ -tetrachloro-1,1′ -3,3′ -tetraethylbenzimidazolcarbocyanine iodide (JC