Quinoline-2,8-diol

Boronate Affinity Chromatography

of a wide variety of cis-diol-containing compounds, including catechols, nucleosides, nucleotides, nucleic acids, carbohydrates, glycoproteins, and enzymes ( 2 ) (see Note 1 ). The basic interaction for boronate chromatography is esterification

On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detectio

. Detailed procedures are given for: (1) on column labeling of proteins with a fluorogenic reagent, 5-furoyl quinoline-3- carboxaldehyde, (2) CE separation of labeled proteins, (3) preparation of a protein extract from cultured cells, and (4) manipulations

Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Derivatization and Laser-Induced Fluorescence Detection

, the protein extracts are obtained by cell lysis using sonication. The water-soluble fraction of these lysates is derivatized on-capillary with the fluorogenic reagent 3-(2-furoyl)quinoline-2-carboxaldehyde. The fluorescent products are analyzed by CE