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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA, Flow Cyt
- 浓度:
详见COA
- 靶点:
B7-H3
- 抗体英文名:
Anti-B7-H3 (TAA06 biosimilar) mAb
- 抗体名:
Anti-B7-H3 (TAA06 biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | B7-H3 |
| Uniprot ID | Q5ZPR3 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | TAA06 |
| 货期 | In Stock |
| 产品英文名称 | Anti-B7-H3 (TAA06 biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature. |
| 别名 | B7H3, CD276, B7 homolog 3 |
| 应用 | ELISA, Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000, Flow Cyt 1:100 |
| 应用范围 | Research use only |
Figure 1. B7-H3 protein is highly expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with 1μg/mL Anti-B7-H3 (TAA06 biosimilar) mAb (BME100181) on HEK293 cells transfected with human B7-H3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram),and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H3 Protein, mFc-His Tag(PME100012) can bind Anti-B7-H3 (TAA06 biosimilar) mAb(BME100181) in a linear range of 0.64–16 ng/mL.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验关键词(如:CD56),并在后方的应用栏选择相应的应用技术,点击搜索即可。界面清晰明了,缺点是收录的抗体供应商相对较少(约85家)。 05 Antibodyresource 网址:https://www.antibodyresource.com/ Antibodyresource(www.antibodyresource.com)上,可以非常简便地查找抗体和ELISA试剂。 06 Antibody Validation Database 网址:http://compbio.med.harvard
人B7-H4 ( B7-H4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 B7-H4 单抗包被于酶标板上,标准品和样品中的 B7-H4与单抗结合,加入生物素化的抗人 B7-H4 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止
点击submit进行查询。点击验证的抗体数据库,得到相关信息如下图:点击PCNA抗体,得到相关信息如下图:Labome也能显示厂家的名称,货号等,点击抗体名称AKT后出现该抗体的详细界面,可通过Reference搜索相关参考文献,看这个抗体在别人实验中的效果怎么样。3、Antibody Validation Databasehttp://compbio.med.harvard.edu/antibodies/Antibody Validation Database也是一个比较专业的抗体搜索引擎。以H3
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