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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Homo sapiens
- 应用范围:
ELISA; Flow Cyt
- 浓度:
详见COA
- 靶点:
Nectin-4
- 抗体英文名:
Anti-Nectin-4(enfortumab biosimilar) mAb
- 抗体名:
Anti-Nectin-4(enfortumab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | Nectin-4 |
| Uniprot ID | Q96NY8 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. Our unconjugated biosimilar monoclonal antibodies (mAbs) are based on the sequences outlined in relevant patents or scientific publications. These antibodies are in their native, unconjugated form, meaning they do not contain any payload or therapeutic agent attached. They are designed for use in research and development, and their performance has been tested as standalone molecules through comprehensive QC tests. |
| 通用名 | enfortumab, Unconjugated mAb |
| 货期 | In Stock |
| 产品英文名称 | Anti-Nectin-4(enfortumab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | EDSS1;LNIR;nectin-4;PRR4;PVRL4 |
| 应用 | ELISA; Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Homo sapiens |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag PME100874 can bind Anti-Nectin-4 Neutralizing antibody ( BME100088) in a linear range of 0.61–39.06 ng/mL.

Figure 2. Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (BME100088) on HEK293 cells transfected with Human Nectin-4 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 3. Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb(BME100088). (A) BME100088 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of BME100088 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of BME100088 to Nectin-4. Antibodies were incubated at 2 μg/mL.
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,随后研究发现,其在多种肿瘤组织中出现异常表达,包括尿路上皮癌、乳腺癌、肺癌以及胰腺癌等 [10]。 相比正常成人组织,部分肿瘤细胞中Nectin-4表达明显升高,同时其位于细胞表面的特点使其具有较好的抗体可及性。这些特征使Nectin-4成为抗体药物开发的重要候选靶点。 目前最具代表性的应用是靶向Nectin-4的抗体偶联药物(antibody-drug conjugate, ADC)——Enfortumab vedotin。该药物利用抗Nectin-4抗体识别肿瘤细胞表面的Nectin
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不只是 PD-1:CD96 正成为免疫治疗的下一个“必争之地”
CD8+ T 细胞表面交联 CD96 时,可诱导细胞活化、效应细胞因子产生以及增殖 [11]。这一激活作用主要通过 MEK-ERK 通路传导 [11]。通过该通路,CD96 能促进 NUR77 和 T-bet 等转录因子的表达,并增强 CD8+ T 细胞的细胞毒性效应,进而影响效应 T 细胞分化 [11]。 相关体内研究进一步表明,无论是抗体阻断 CD96 活性还是基因敲除 CD96,均会损害 CD8+ T 细胞的活化和效应功能 [11]。这说明,在特定细胞类型中,CD96 并非单纯抑制性受体
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