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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG2 - Kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Homo sapiens
- 应用范围:
ELISA, Flow Cyt
- 浓度:
详见COA
- 靶点:
B7-H2
- 抗体英文名:
Anti-B7-H2(prezalumab biosimilar) mAb
- 抗体名:
Anti-B7-H2(prezalumab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | B7-H2 |
| Uniprot ID | O75144 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | AMG557, AMG-557, MEDI-5872 |
| 货期 | In Stock |
| 产品英文名称 | Anti-B7-H2(prezalumab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | ICOSLG;B7-H2;B7H2;B7RP-1;B7RP1;CD275;GL50;ICOS-L;ICOSL;LICOS;ICOS ligand |
| 应用 | ELISA, Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Homo sapiens |
| IgG亚型 | Human IgG2 - Kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000, Flow Cyt 1:100 |
| 应用范围 | Research use only |
Figure 1. Flow cytometry analysis with 15 μg/mL Anti-B7-H2 (prezalumab biosimilar) mAb (BME100081) on HEK293 cells transfected with Human B7H2 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H2 Protein, mFc-His Tag (PME100029) can bind Anti-B7-H2(prezalumab biosimilar) mAb (BME100081) in a linear range of 40–200 ng/mL.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
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当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
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