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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Homo sapiens
- 应用范围:
ELISA; Flow Cyt
- 浓度:
详见COA
- 靶点:
CD40
- 抗体英文名:
Anti-CD40 (iscalimab biosimilar) mAb
- 抗体名:
Anti-CD40 (iscalimab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | CD40 |
| Uniprot ID | P25942 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | CFZ-533, NVP-CFZ533, OM11-62MF |
| 货期 | In Stock |
| 产品英文名称 | Anti-CD40 (iscalimab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | CD40;Bp50;CDW40;MGC9013;TNFRSF5;p50 |
| 应用 | ELISA; Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Homo sapiens |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 应用范围 | Research use only |
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40, mFc-His tagged protein ([getskuurl sku="PME100015"]) can bind Anti-CD40 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml.
Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human CD40 protein (A) were surface stained with anti-CD40 neutralizing antibody 1μg/ml (iscalimab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CD40 分子 CD40 常用单克隆抗体或代号: G28- 5,EA- 5 主要表达细胞: B,M,FDC,并指细胞,上皮 [B] 分子质量(kDa)和结构: gp50(TNFR- SF) 功 能: B细胞生长、分化和记忆细胞产生,配体为CD40L,T- B相互作用 CD40 Plays a central role in regulating cell-mediated
(3)Th细胞:T-B细胞相互作用不仅决定B细胞的激活和对TD抗原的抗体应答,也与抗体的类别转换有关。例如T细胞CD40L基因突变可形成X性联高IgM综合征,患者生发中心的发育严重受阻,也无法启动抗体的类别转换,IgG类抗体的缺如,使患者抗胞外菌感染的能力明显下降,症状多少类似于XLA。因为CD40L-CD40的配接可在B细胞中激活NF-κB等转录因子,诱导类别转换。敲除CD40/CD40L基因的小鼠,也显示类别转换严重受阻,同时伴有亲和力成熟障碍和记忆B细胞生成不良。 3.细胞因子直接调节抗体类别
1.留存抗体和桨细胞 抗原清除后,体内留存的抗体可特异性地直接结合再次出现的病毒颗粒、细菌和寄生虫,对后者实施中和作用和调理功能,构成记忆性抗感染的第一道防线,但发挥效应的时间不长。 抗体由浆细胞产生。浆细胞的寿命通常只有几天,称为短寿性浆细胞短寿性浆细胞系初始B细胞由抗原直接诱导产生,停留在外周淋巴器官和组织。长寿性浆细胞则经由生发中心由记忆性B细胞产生。近年来的研究揭示,此类浆细胞可长期存活。 2.记忆性B细胞 初始B细胞遭遇TD抗原后,在Th
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