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文献和实验C oven (Horvitz lab: this is in the autoclave room). Plasticware (pipette tips, centrifuge tubes, 10 ml disposable pipettes) should be from an unopened package that is labelled RNase free and then kept in a special drawer after opening
秀丽线虫RNA提取--C. elegans RNA prep
disposable tube. It should be brownish yellow. 6. You will need to prepare one cesium gradient for each 6 mls of homogenate. Use RNase free 16X102 mm polyallomar tubes, which fit the SW28.1 buckets (which in the Horvitz lab we spin on an SW28 rotor). Don
Micropipettors (e.g., Gilson Pipetman P10, P20, P200, P1000) and disposable barrier tips (sterile, DNase/RNase-free) Microscope (inverted, equipped with bright-field optics) Pipettes (sterile serological; 2-, 5-, 10-, and 25-mL) Plates, dishes
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