Sterile High Binding 96-well M
icroplate with Lid, Individual Package, Clear Flat Bottom{96孔透明平底酶标板，高等吸附性，无酶无菌，带盖独立盒装}- ￥120 - 1200
- ABclonal
- AI04002
- 2026年07月14日
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10 pcs/100 pcs
| 规格： | 10 pcs | 产品价格： | ￥120.0 |
|---|---|---|---|
| 规格： | 100 pcs | 产品价格： | ￥1200.0 |
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文献和实验Two-hybrid analysis of genetic
X-Gal Replica plater and sterile velvets for 150 mm diameter plates.(A replica devise can be fashioned from a box of 200 µl pipet tips by stretching a velvet over the top of the box) 96-prong device (e.g.DanKar MC-96)with 3 mm diameter flat ended metal
101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
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