Biotinylated Recombinant Cynom

BioID: A Screen for Protein‐Protein Interactions

expressing cells. (4 ) Induce biotinylation with excess biotin prior to (5 ) cell lysis and protein denaturation. (6 ) Perform biotin‐affinity capture of biotinylated proteins. (7 ) Reserve 10% of the sample for streptavidin immunoblot analysis. Use

Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays

are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides

Identification of Protein Interactions by Far Western Analysis

Recombinant protein or unlabeled in vitro–translated protein for probe 5% (w/v) non‐fat instant dry milk in recipe1× TBST (see recipe for recipeTBST