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- CAS号：
1256094-72-0
- 规格：
1mLx10mM(inDMSO)/1mg/5mg/10mg/25mg/50mg/100mg/200mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥473.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥189.0 |
| 规格： | 5mg | 产品价格： | ￥325.0 |
| 规格： | 10mg | 产品价格： | ￥513.0 |
| 规格： | 25mg | 产品价格： | ￥1032.0 |
| 规格： | 50mg | 产品价格： | ￥1544.0 |
| 规格： | 100mg | 产品价格： | ￥2216.0 |
| 规格： | 200mg | 产品价格： | ￥3104.0 |
Product Introduction
Bioactivity
| 名称 | AI-10-49 |
| 描述 | AI-10-49 is a selective inhibitor of the binding of CBFβ-SMMHC to RUNX1 with IC50 of 260 nM. |
| 细胞实验 | Cell lines are cultured in IMDM supplemented with 10%~20% fetal bovine serum (FBS) according to culture conditions indicated by ATCC and 1% penicillin/streptomycin (Pen/Strep). All cell lines are tested for mycoplasma. Cells are cultured at 300,000 cells per ml in 96 well plates for 24 and 48 hours in DMSO, or different of AI-10-47, AI-410-49, AI-4-57, or AI-4-88); each in duplicate or triplicate. Cell viability is evaluated using DAPI by flow cytometry. Data is analyzed using FlowJo software and Graphpad Prism software.(Only for Reference) |
| 激酶实验 | FRET assays: Cerulean-Runt domain is expressed and purified. Venus-CBFβ-SMMHC is constructed by inserting 6xHis tag and Venus into pET22b vector between NdeI and NcoI sites, and by inserting CBFβ-SMMHC (the CBFβ-SMMHC construct contains 369 amino acids, 1-166 from CBFβ and 166-369 from MYH11 (amino acids 1526-1730)) between the NcoI and BamHI sites. The fusion protein is purified by standard Ni-affinity chromatography with an on column benzonase treatment to remove residual DNA contaminants. Proteins are dialyzed into FRET buffer (25 mM Tris-HCl, pH 7.5, 150 mM KCl, 2 mM MgCl2) prior to use. Protein concentrations are determined by UV absorbance of the Cerulean and Venus at 433 and 513 nm, respectively. Cerulean-Runt domain and Venus-CBFβ-SMMHC were mixed 1:1 to achieve a final concentration of 10 nM in 96 well black COSTAR plates. DMSO solutions of compounds are added to a final DMSO concentration of 5% (v/v) and the plates incubated at room temperature for one hour in the dark. A PHERAstar microplate reader is used to measure fluorescence (excitation at 433 nm and emission measured at 474 and 525 nm). For IC50 determinations, the ratios of the fluorescence intensities at 525 nm and 474 nm are plotted versus the log of compound concentration, and the resulting curve was fit to a sigmoidal curve using Origin7.0. Three independent measurements are performed and their average and deviation are used for IC50 data fitting. |
| 体外活性 | AI-10-49对带有inv(16)的ME-1细胞系显示出特异性的生长抑制作用。AI-10-49选择性地与CBFβ-SMMHC结合，打断其与RUNX1的结合，并恢复RUNX1的转录活性。[1] |
| 体内活性 | 在转移了Cbfb+/MYH11;Nras+/G12D白血病细胞的小鼠中，AI-10-49（200 mg/kg，i.p.）可减缓白血病细胞扩散。[1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | Ethanol : 93 mg/mL (140.8 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (5 mM), Sonication is recommended. DMSO : 93 mg/mL (140.8 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble) |
| 关键字 | RNASynthesis | RNA Synthesis | Inhibitor | inhibit | DNASynthesis | DNA Synthesis | CBFβ-SMMHC | AI-10-49 | AI1049 | AI 10 49 |
| 相关产品 | Carbazole | Hexane-1,6-diol | Guanidine hydrochloride | Vidarabine | 1,4-Naphthoquinone | 5-Fluorouracil | Usnic Acid | Adenine hemisulfate | Erythromycin thiocyanate | Adenine | Docosanoic acid | Thymidine |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Fluorochemical Library | Anti-Aging Compound Library | NO PAINS Compound Library | DNA Damage & Repair Compound Library |
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网络 第十九章 杂环化合物和生物碱 杂环化合物在自然界分布很广，其数量几乎占已知有机化合物的三分之一，用途也很多。许多重要的物质如叶绿素、血红素、核酸以及临床应用的一些有显著疗效的天然药物和合成药物等，都含有杂环化合物的结构。生物碱多是中草药的有效成分，绝大多数是含氮的杂环化合物。本章内容与医学关系密切，具有重要意义。
分子中具有高能键的化合物。在生物体内主要有上式（Ⅰ）和（Ⅱ）两种结构的高能化合物。其作用为贮藏能量（例如 ATP、肌酸磷酸）和作为产生 ATP源的中间代谢产物（例如磷酸烯醇丙酮酸）以及合成反应的中间物质（例如酰基辅酶 A），在蛋白质的结构（ conformation）变化周期的第一阶段上（例如钠钾 ATP酶的羧基磷酸中间体）也具有重要作用。多数高等化合物具有磷酸基，但也有例外。
由不同种元素组成的纯净物叫做化合物。化合物一般有固定的组成。化合物的组成一般可用化学式表示。化合物具有确定的物理性质和化学性质，不同于其组成元素的性质。化合物中的元素不能用简单的机械方法或物理方法分开，而必须用化学方法才能分离。
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化合物 AI-10-49【1256094-72-0】
￥189 - 3104
