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1mg/5mg/10mg
| 规格: | 1mg | 产品价格: | ¥1124.0 |
|---|---|---|---|
| 规格: | 5mg | 产品价格: | ¥3334.0 |
| 规格: | 10mg | 产品价格: | ¥5317.0 |
Product Introduction
Bioactivity
| 名称 | Anti-Monkey/Human CD40L/CD154 Antibody (5C8) |
| 描述 | Anti-Monkey/Human CD40L/CD154 Antibody (5C8) is a mouse inhibitory antibody targeting monkey/human CD40L/CD154, blocking the interaction between CD154 and CD40. It can be used in inflammation and immunology-related research, including colitis and transplantation studies. |
| 体外活性 | Anti-Monkey/Human CD40L/CD154 Antibody (5C8)在 0.01–100 μg/mL 浓度范围内,可对恒河猴混合淋巴细胞反应产生抑制作用 [1]。 |
| 体内活性 | Anti-Monkey/Human CD40L/CD154 Antibody (5C8) 以20 mg/kg剂量静脉注射,每两天给药一次、连续 14 天,能够抑制肾移植小鼠模型中的同种异体肾移植排斥反应[1]。 Anti-Monkey/Human CD40L/CD154 Antibody (5C8) 给予250 μg / 只, 腹腔注射,每周两次、持续 8 周,可预防 CD45RBhigh CD4+ T 细胞重建小鼠模型中实验性结肠炎的发生并缓解病情[2]。 |
| 存储条件 | Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature. |
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文献和实验) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
、ionomycin和Brefeldin A,其中前两者协同激活细胞,后者抑制蛋白转运,从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体,易非特异性结合抗体的Fc段,导致假阳性信号。为解决此问题,菲恩生物提供Purified Anti-Mouse CD16/32 Antibody(FNab30106)用于小鼠样本封闭,;Human Fc Receptor Blocking reagent(K128)用于人样本封闭。关键在于必须选择与样本
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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