- ￥2600
- Proteintech
- 15.6 - 500 pg/mL
- KE00095
- 2026年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
15.6 - 500 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatants
- 规格：
96T
Product Information
KE00095 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human leptin ELISA kit is to be used to detect and quantify protein levels of endogenous human . The assay recognizes human leptin. An antibody specific for human leptin has been pre-coated onto the microwells. The human leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human leptin is added to detect the captured human leptin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Leptin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 15.6 - 500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3952 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 82%-113% |
| Cell culture supernatants | 98% | 80%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 79.7 | 2.8 | 3.5 |
| 2 | 20 | 182.7 | 5.8 | 3.2 |
| 3 | 20 | 461.7 | 20.9 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 84.3 | 1.9 | 2.2 |
| 2 | 24 | 185.1 | 4.6 | 2.5 |
| 3 | 24 | 461.7 | 15.4 | 3.3 |
Background Information
Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LEP, OB, Obese protein, Obesity factor, OBS |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人瘦素 (Leptin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Leptin 单抗包被于酶标板上，标准品和样品中的 Leptin与单抗结合，加入生物素化的抗人
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人瘦素受体 (Leptin R)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Leptin R 单抗包被于酶标板上，标准品和样品中的 Leptin R与单抗结合，加入生物
人瘦素（leptin ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 瘦素（leptin ） 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人瘦素（ leptin ）水平。用纯化的人瘦素（ leptin ）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 瘦素（ leptin ） ，再与 HRP 标记的瘦素
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