- ￥2600
- Proteintech
- 62.5-4000 pg/mL
- KE00033
- 2026年07月14日
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- 文献和实验
- 技术资料
- 检测范围：
62.5-4000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma
- 规格：
96T
Product Information
KE00033 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HDAC1 ELISA kit is to be used to detect and quantify protein levels of endogenous HDAC1. The assay recognizes human HDAC1. An antibody specific for HDAC1 has been pre-coated onto the microwells. The HDAC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HDAC1 is added to detect the captured HDAC1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HDAC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 86%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,406.1 | 197.3 | 8.2 |
| 2 | 20 | 499.5 | 19.9 | 4.0 |
| 3 | 20 | 163.5 | 6.3 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,282.9 | 158.9 | 7.0 |
| 2 | 24 | 484.8 | 24.5 | 5.1 |
| 3 | 24 | 157.3 | 6.9 | 4.4 |
Background Information
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/ acuc/ apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. HADC inhibition causes apoptosis in tumor cells and HDAC inhibitors may be developed as anti-cancer agents. This kit is used to quantify HDAC1 level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1, histone deacetylase 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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