- ￥2600
- Proteintech
- 0.156-10 ng/mL
- KE01112
- 2026年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
0.156-10 ng/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human/Mouse
- 样本：
Cell lysate, Tissue lysate
- 规格：
96T
Product Information
KE01112 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse NKCC1/SLC12A2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse NKCC1/SLC12A2. The assay recognizes human/mouse NKCC1/SLC12A2. An antibody specific for human/mouse NKCC1/SLC12A2 has been pre-coated onto the microwells. The human/mouse NKCC1/SLC12A2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse NKCC1/SLC12A2 is added to detect the captured human/mouse NKCC1/SLC12A2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse NKCC1/SLC12A2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6558 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 96%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.21 | 0.27 | 5.18 |
| 2 | 8 | 1.31 | 0.04 | 3.05 |
| 3 | 8 | 0.63 | 0.03 | 4.76 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.13 | 0.32 | 6.24 |
| 2 | 16 | 1.22 | 0.11 | 9.02 |
| 3 | 16 | 0.64 | 0.03 | 4.69 |
Background Information
The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface. The abundant expression of NKCC1 protein in various types of cancer cells, such as gastric cancer cells, prostate cancer cells, and brain tumor cells, which is associated with tumor cell proliferation, migration, and invasion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验抗体的轻、重链嵌合基因共转染受体细胞 目前将重组子导入宿主细胞主要采用的方法有磷酸钙沉淀法、DEAE-葡聚糖街道法介导法、聚季铵盐介导法、原生质体融合法、电转染法以及微注射法。其中以电转染效率较高。 【材料和试剂】 （1） 小鼠骨髓瘤细胞系Sp2/0细胞或P3X63.Ag8.653细胞，或CHO细胞。 （2） 已构建的轻、重链嵌合基因质粒DNA。 （3） 脂质体。 （4） 电转
37℃过夜，余细胞同前。 4．杂交后漂洗2×SSc 5min×3，1×SSc 1min室温，0.5×SSc 55℃15min×2，室温漂洗于0.5×SSc 3min×2。梯度酒精脱水，切片室温干燥。 5．浸核乳胶切片浸入核乳胶，黑盒封闭，在4℃曝光2～3周（视同位素种类），显影，复染，观察。 相关链接： 仪器库 >> 显微系统 >> 倒置显微镜 仪器库 >> 影像系统 >>专业影像系统 试剂库 >> 免疫检测 >>ELISA检测试剂盒
、自动小夹、带弯针4/0丝线及持针器、直径为10cm塑料 Petri氏培养皿（内盛70%乙醇）。2．手术前麻醉2，2，2 三溴乙醇（阿佛丁）是相当有效的麻醉剂。因该试剂对光敏感，所以应该于4度避光保存，新试剂用前应进行测试。经腹腔注射三溴乙醇麻醉小鼠后，为了使麻醉剂尽快发生作用，将小鼠放回原笼熟悉的环境中，小鼠将会更容易松驰下来。 挤压小鼠脚垫，如果小鼠能感觉到此刺激，小鼠将从挤压部位开始缩腿运动（pedal reflex），用此方法可以检察是否麻醉完全。实验中小鼠无缩腿运动、小鼠达到手术所需的麻醉
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