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- 文献和实验
- 技术资料
- 检测范围:
39-2500 pg/mL
- 应用:
Sandwich ELISA
- 适应物种:
Human
- 样本:
Serum, Plasma
- 规格:
96T
Product Information
| Product name | Speedy™ Human Coagulation factor II One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注:对未验证样本,我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2147 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 109% | 98%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,224.0 | 47.0 | 3.8 |
| 2 | 8 | 301.0 | 9.5 | 3.2 |
| 3 | 8 | 153.2 | 5.9 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,190.0 | 48.3 | 4.1 |
| 2 | 16 | 294.4 | 11.3 | 3.8 |
| 3 | 16 | 151.8 | 4.8 | 3.2 |
Background Information
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Coagulation Factor II is a vitamin K-dependent glycoprotein that is primarily synthesized in the liver and secreted into circulating plasma. It is also the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, EC:3.4.21.5 |
FAQ
1、我的样本稀释液有限,如何处理稀释倍数较大的样本?
我们建议您对样本进行梯度稀释。例如,人血清样本需要1000000倍稀释时,您可以分3个梯度,每个梯度100倍,进行稀释:
第一步:取2 uL人血清样本,加入198 uL样本稀释液,混匀得到100倍稀释的人血清样本
第二步:取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到10000倍稀释的人血清样本
第三步:取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释,您可以使用更多梯度。除了我们推荐的样本稀释液,不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足,请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器,这会对实验时间和结果产生影响吗?
如果您没有恒温振荡器,完成这个实验大约需要 2.5 小时。在加入抗体混合物后,我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后,您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明,尽管信号会略有降低,但您仍然可以获得有效的实验数据。
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文献和实验Use of Human Hepatocytes to Investigate Blood Coagulation Factor
Coagulation is the complex process by which activation of plasmatic haemostasis proteins ends up with the generation of fibrin. Most of the plasma coagulation proteins are synthesized in hepatocytes. The aim of this chapter
血液凝固因子 blood coagulation factor
血液凝固因子 blood coagulation factor 指直接参与血液凝固机制的各种因子,亦称血凝因子或血浆凝固因子。自 P. Morawitz( 1903)研究凝血活素以后,由于研究的进步,各种因子相继发现,为避免命名混乱,成立了国际血凝因子名称审定委员会。该委员会提议对一般已承认的血凝因子附以罗马数字来统一命名。继 1954年后,在 1959年第四次委员会上制定了如下页表所示名称。这里第Ⅺ因子和第Ⅻ因子,亦称接触因子( con- tact factor)。
Disseminated intravascular coagulation (DIC) is a complex acquired, coagulopathy resulting from excessive thrombin formation. Abnormal tissue factor (TF) expression is a major mechanism initiating DIC in many disorders, including obstetric
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