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- 文献和实验
- 技术资料
- 检测范围:
78.1-5000 pg/mL
- 应用:
Sandwich ELISA
- 适应物种:
Mouse
- 样本:
Serum, Plasma, Cell culture supernatant, Urine
- 规格:
96T
Product Information
KE10202 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Fetuin A/AHSG ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Fetuin A/AHSG. The assay recognizes mouse Fetuin A/AHSG. An antibody specific for mouse Fetuin A/AHSG has been pre-coated onto the microwells. The mouse Fetuin A/AHSG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Fetuin A/AHSG is added to detect the captured mouse Fetuin A/AHSG protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Fetuin A/AHSG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine 备注:对未验证样本,我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11625 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 84%-99% |
| Urine | 100% | 95%-108% |
| Cell culture supernatant | 93% | 79%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,440.0 | 95.0 | 3.9 |
| 2 | 8 | 612.2 | 17.6 | 2.9 |
| 3 | 8 | 340.7 | 13.4 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,490.5 | 99.3 | 4.0 |
| 2 | 16 | 602.4 | 20.1 | 3.3 |
| 3 | 16 | 330.3 | 17.9 | 5.4 |
Background Information
Fetuin-A, also named Alpha2-Heremans-Schmid glycoprotein (AHSG), is a 52 kDa glycoprotein synthesized in hepatocytes and secreted into the bloodstream: it is therefore addressed as a hepatokine. It acts as systemic inhibitor of extraosseous calcification, serves as transport protein for calcium and phosphate in colloidal calciprotein particles and is involved in bone mineralization. Fetuin-A is its interaction with the insulin receptor. Fetuin-A has also been identified as a biomarker for neurodegenerative disease. Fetuin-A modulation is associated with a number of pathophysiological variables that cause liver problems, including insulin receptor signaling deficiencies, adipocyte dysfunction, hepatic inflammation, fibrosis, triacylglycerol production, macrophage invasion, and TLR4 activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ahsg, Alpha-2-HS-glycoprotein, Countertrypin, Fetua, Fetuin-A |
FAQ
1、我的样本稀释液有限,如何处理稀释倍数较大的样本?
我们建议您对样本进行梯度稀释。例如,人血清样本需要1000000倍稀释时,您可以分3个梯度,每个梯度100倍,进行稀释:
第一步:取2 uL人血清样本,加入198 uL样本稀释液,混匀得到100倍稀释的人血清样本
第二步:取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到10000倍稀释的人血清样本
第三步:取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释,您可以使用更多梯度。除了我们推荐的样本稀释液,不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足,请联系我们的客服为您提供更多样本稀释液。
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