- ￥2600
- Proteintech
- 15.6-1000 pg/mL
- KE00549
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
15.6-1000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatant, Cell lysate
- 规格：
96T
Product Information
KE00549 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human EPCAM/CD326 ELISA kit is to be used to detect and quantify protein levels of endogenous human EPCAM/CD326. The assay recognizes human EPCAM/CD326. An antibody specific for human EPCAM/CD326 has been pre-coated onto the microwells. The human EPCAM/CD326 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human EPCAM/CD326 is added to detect the captured human EPCAM/CD326 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EPCAM/CD326 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4072 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 79%-101% |
| Cell culture supernatant | 105% | 100%-109% |
| Cell lysate | 109% | 100%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 558.1 | 52.1 | 9.3 |
| 2 | 8 | 134.3 | 7.5 | 5.6 |
| 3 | 8 | 36.8 | 2.5 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 541.7 | 49.7 | 9.2 |
| 2 | 16 | 125.1 | 12.7 | 10.1 |
| 3 | 16 | 34.7 | 3.2 | 9.3 |
Background Information
Epithelial cell adhesion molecule (EPCAM), also known as CD326, is a well-characterized type I transmembrane glycoprotein that plays a pivotal role in cell adhesion, proliferation, differentiation, and signaling. It mediates Ca2+-independent homotypic cell-cell adhesion through its extracellular domain, which is crucial for the formation and maintenance of epithelial tissues. The membrane localization of EPCAM is essential for its function in cell adhesion and signaling. Additionally, elevated levels of soluble EPCAM have been associated with tumor burden and poor prognosis in cancer patients, making it a potential biomarker for cancer diagnosis and monitoring.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPCAM,Adenocarcinoma-associated antigen,CD326,Cell |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验+ - GICA + 178 6 184 - 5 137 142 合计 183 143 326 由表1可见，若以ELISA检测抗Hp-CagA抗体试剂盒作为参照标准，则GICA法的特异性为95.8%，敏感度为97.3%，总符合率为96.6%。 3、稳定性试验 GICA试剂盒于4℃下放置6 mo，每月检测抗Hp-CagA IgG阳性及阴性血清各50份，该试剂盒的各种性能指标均稳定。 将GICA试条于37℃温箱放置6 d，做
+ - GICA + 178 6 184 - 5 137 142 合计 183 143 326 由表1可见，若以ELISA检测抗Hp-CagA抗体试剂盒作为参照标准，则GICA法的特异性为95.8%，敏感度为97.3%，总符合率为96.6%。 3、稳定性试验 GICA试剂盒于4℃下放置6 mo，每月检测抗Hp-CagA IgG阳性及阴性血清各50份，该试剂盒的各种性能指标均稳定。 将GICA试条于37℃温箱放置6 d，做
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