- ￥2600
- Proteintech
- 15.6-1000 pg/mL
- KE00611
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
15.6-1000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma
- 规格：
96T
Product Information
KE00611 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human E-selectin / CD62E ELISA kit is to be used to detect and quantify protein levels of endogenous human E-selectin / CD62E. The assay recognizes human E-selectin / CD62E. An antibody specific for human E-selectin / CD62E has been pre-coated onto the microwells. The human E-selectin / CD62E protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human E-selectin / CD62E is added to detect the captured human E-selectin / CD62E protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human E-selectin / CD62E ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6401 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 103%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 447.8 | 19.4 | 4.3 |
| 2 | 8 | 119.4 | 1.6 | 1.4 |
| 3 | 8 | 60.8 | 3.8 | 6.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 452.7 | 16.1 | 3.6 |
| 2 | 16 | 116.5 | 4.1 | 3.5 |
| 3 | 16 | 59.1 | 5.1 | 8.6 |
Background Information
E-selectin (Endothelial Leukocyte Adhesion Molecule-1, also known as CD62E) is a critical adhesion molecule expressed on endothelial cells. It plays a crucial role in the initial steps of leukocyte recruitment to sites of inflammation, facilitating the rolling and adhesion of leukocytes to the endothelium. This process is essential for the immune response to infection and injury. E-selectin is upregulated in response to inflammatory cytokines such as IL-1, TNF-α, and bacterial lipopolysaccharide (LPS), and it interacts with various glycan ligands on leukocytes, including PSGL-1, ESL-1, CD44, and CD43.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验在细胞表面） CD15 E-selectin(CD62E,ELAM-1) 血管内皮细胞（主要在毛细血管后静脉，IL-1，TNF活化后表达） 115 S-Lewis x S-Lewis x CLA 注： LECAM:leukocyte endothelial cell adhesion molecule,白细胞内皮细胞粘附分子
E-Z 96 X-press Plasmid Vacuum Protocol
well plate inside the manifold base; 3) Place the top plate of manifold over the base, the deep well plate or waste collection tray now should be positioned under the E-Z 96 X-press Plate. Seal the unused wells of E-Z 96 Xpress Plate with sealing
E-Z 96 X-press Plasmid Spin Protocol
-well plate. Transfer the cell lysate to each well of E-Z 96 X-press plate. 3. Centrifuge at 3000 x g for 5 minutes at room temperature. Discard the flow-through and re-use the deep well plate. 4. Add 500μl DLW Buffer (diluted with isopropanol
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