- ￥2600
- Proteintech
- 15.6-1000 pg/mL
- KE10220
- 2026年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
15.6-1000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Mouse
- 样本：
Serum, Plasma, Cell culture supernatant, Tissue lysate, Tissue homogenate
- 规格：
96T
Product Information
KE10220 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGF. The assay recognizes mouse VEGF. An antibody specific for mouse VEGF has been pre-coated onto the microwells. The mouse VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse VEGF is added to detect the captured mouse VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VEGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate, Tissue homogenate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.9 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22339 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 93% | 92%-94% |
| Tissue lysate | 94% | 93%-96% |
| Tissue homogenate | 97% | 91%-104% |
| Mouse serum | 77% | 72%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 520.7 | 22.0 | 4.2 |
| 2 | 8 | 140.3 | 9.0 | 6.4 |
| 3 | 8 | 67.9 | 3.7 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 547.4 | 44.8 | 8.2 |
| 2 | 16 | 141.6 | 8.3 | 5.9 |
| 3 | 16 | 72.0 | 5.2 | 7.2 |
Background Information
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | VEGFA, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, Vascular endothelial growth factor A, long form |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠血管内皮生长因子 ( VEGF )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 VEGF 单抗包被于酶标板上，标准品和样品中的 VEGF与单抗结合，加入生物素化的抗小鼠
小鼠血管内皮生长因子-A(VEGF-A)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠血管内皮生长因子-A ( VEGF-A )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 VEGF-A 单抗包被于酶标板上，标准品和样品中的 VEGF-A与单抗结合
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